led1Buffer_555 =  22625.67
led1Sample_555 = 16093
led2Buffer_427 = 13511.33
led2Sample_427 = 2714.33

import math

absorbance = - math.log(led1Buffer_555/led1Sample_555) / - math.log(led2Buffer_427 / led2Sample_427)

print("absorbance", absorbance)