function param = parameter(image)
    t1 = toc;
    bw = rgb2gray(image);
    [Gx, Gy] = imgradientxy(bw);
    %[Gmag, Gdir] = imgradient(Gx, Gy);
    gradImage = rgb2gray(imfuse(Gx,Gy));
    %temp = histeq(gradImage);
    threshImage = im2bw(gradImage,graythresh(gradImage));
    [~, radii] = imfindcircles(threshImage,[10 60]);
    cell_area = round(sum(3.14*(radii.^2)));
    bg = rgb2gray(imread('bg.jpg'));
    a = histeq(bw)-bg;
    level2 = graythresh(a);
    bgImg = bwareaopen(im2bw(a,level2),800);
    bg_pixels = sum(sum(bgImg == 1));
    fg_pixels = (1280 * 1024) - bg_pixels;
    fg_pixels = fg_pixels - round(0.1*fg_pixels);
    param = cell_area/fg_pixels;
    t2 = toc;
    disp(t2-t1)
end